![]() ![]() This allows a much better separation of the components of the mixture. It also allows you to use a very much smaller particle size for the column packing material which gives a much greater surface area for interactions between the stationary phase and the molecules flowing past it. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres. High performance liquid chromatography is basically a highly improved form of column chromatography. Use the BACK button on your browser to return quickly to this page. HPLC is essentially an adaptation of column chromatography - so it might be a good idea to have a (very quick) look at that as well. High performance liquid chromatography works on the same basic principle. Note: It is important to read the introductory page about thin layer chromatography before you continue with this one - particularly the part about how thin layer chromatography works. This page looks at how it is carried out and shows how it uses the same principles as in thin layer chromatography and column chromatography. High performance liquid chromatography is a powerful tool in analysis. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - HPLC ![]() High performance liquid chromatography - hplc ![]()
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